Preventing clogs

Question

Hi Everyone, 
 I am running a buffer composed of 25mM Tris, 1mM EDTA, 1M NaCl, pH 8.0 through an 1260 Infinity 2 Quaternary System for single strand DNA purification. The column is anion exchange from waters. What can I use to make sure the salts don't clog the instrument? 
 -Thank you

gabcaron · Accepted Answer

Sounds like you might have had small leaks during your runs so buffer leaked out and eventually liquid evaporate and only the salts were left. I would make sure i have no leaks and clean the system with water regularly. You might have had a partial clog at some point and it forced liquid out.

Preventing clogs

Top Replies