FLD Flow Cell Pathlength (G1321-60005)

Hello,

I am using a G1321-60005 flow cell in a G1321B FLD detector. I was wondering if I might get information on the pathlengths for the light entering and exiting the cell? I have an application where I would like to apply inner filter corrections to the data.

Thanks!

Stacey

  • Hi,

    As per manual light path is as per this pic.

  • Hello Stacey,

    If I understand you correctly, you are asking for the distances between the EX grating to the cell and from the cell to the EM grating, correct?  We don't have those distances specified.  The term "pathlength" is used to define the distance of the light path within a flow cell and only relevant to UV flow cells to compensate for signal responses between flow cells with different path lengths because of Beer-Lambert law.  There are no specifications for cell pathlength in FL since they don't follow Beer-Lambert's Law.

  • Of course, there are drawings of the G1321-60005 8ul cell, but they're confidential. Honestly, so far I did not come across an application, where such detail was required.

  • Hello all, thank you for the responses!

    To clarify, I am interested in the liquid path length within the flow cell. The analysis I am aiming to try is an "inner filter" correction to correct the measured fluorescence intensities for light absorbed entering the cell at the excitation wavelength and exiting at the emission wavelength. An application of this correction is to help distinguish true fluorescence quenching from absorption.

    I would imagine the cell dimensions would not be confidential as it seems to be standard information for optical measurements? 

  • Yes, for UV detection this is normal, but an FLD cell is not just a block of a rectangle shape, where you just measure from entrance to exit.

  • Hello all, thank you for the responses!

    To clarify, I am interested in the liquid path length within the flow cell. The analysis I am aiming to try is an "inner filter" correction to correct the measured fluorescence intensities for light absorbed entering the cell at the excitation wavelength and exiting at the emission wavelength. An application of this correction is to help distinguish true fluorescence quenching from absorption.

    I would imagine the cell dimensions would not be confidential as it seems to be standard information for optical measurements? 

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