Hi ,sorry to ask a lot of questions .
I want to know which column is best for sugar separation?(fructose, glucose.....)
hi plex ca column?
hi plex k column?
Colonne supelcosil lc NH2?
It depends what you mean by best!
I've used both HiPlex Ca and amino columns like the one you suggest, Both give separations for sugars, employing different separation mechanisms, with quite different mobile phases. If one of the columns doesn't separate all of the sugars you need to work with, maybe the other one will.
Looking at the application notes (https://www.agilent.com/cs/library/applications/5990--8264EN.pdf), the HiPlex H can separate some sugars, but is mainly aimed at small organic acids.