I have a method that no matter what I do I seem to get persistent carryover that doesn't reduce after multiple diluent injections. Has anyone come across materials binding to the rheodyne and then being washed off at a later time? Any suggestions?
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I have a method that no matter what I do I seem to get persistent carryover that doesn't reduce after multiple diluent injections. Has anyone come across materials binding to the rheodyne and then being washed off at a later time? Any suggestions?
Maybe bypass the autosampler, by connecting tubing from the pump directly to the column/column compartment. Perform blank/no injection runs (no samples since there is no flow through the autosampler) If this helps, then the problem is the autosampler. A worn rotor in the autosampler can contribute to carryover.
That was my next question, when is the last time you changed the rotor seal. Like andyg said, worn rotor seal can have some cavities in the grove that retain some of the sample and that gets out in the blank.
Thanks both,
Ill check this out tomorrow when I’m back in the Lab.
Thanks both,
Ill check this out tomorrow when I’m back in the Lab.