How to set Pretreatment Method parameters in Empower for double needle wash

Hi,

 

How to set Pretreatment Method parameters in Empower (with ICF 3.0) to have two-time needle wash for Agilent LC.

When attempting to add the second needle wash, the chromatography shows absolutely no peaks (almost as if it is injection the wash solution instead of sample), and appears as a blank injection. As soon as I removed the wash and only have one time, the chromatography is as expected.

  • Hello,

     

    This might be a question that needs to be asked to Waters. I have not heard of Pretreatment Method with our HPLC and not sure if it is something we can answer. Maybe someone has experience with Empower and they can answer you, but I am not sure.

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