How to rinse and store columns C18, C8. Are there columns we can store only with water?

İ am a new user in chemstation.

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  • HPLC COLUMN PROTECTION
    To save the analytical column Reverse Phase ZORBAX Eclipse Plus C18 (100 x 4,6 mm, 3,5 μ), the use of Javelin Colfilters (1 x 10 pcs.) cod. S90199511 is obligatory.
    HPLC COLUMN CONDITIONING
    Install a new analytical C18 Reversed-Phase column ZORBAX Eclipse Plus C18 (100 x 4,6 mm, 3,5 μ) .
    Disconnect the detector and filter 30 ml of H2O : Acetonitrile (20 : 80 v / v) set flow of 1,0 ml/minute. Don’t recycle the washing solutions. Flux 30 ml of a solution of H2O : Acetonitrile (80 : 20 v / v) at flow of 1,0 ml/minute. Don’t recycle the washing solutions. Condition the column with the mobile phase at a flow of 1,0 ml/minute and discharge the first 20 ml. Finally inject the Chemical Standard and verify the quality of the HPLC run.
    It’s NOT possible to make analysis at recycling phase. If room temperature is  20 °C store the Mobile Phase at 2-8 °C between an analytical session and another.
    COLUMN CLEANING
    Disconnect the detector. Flux 15 ml of H2O : Acetonitrile or Methanol (80 : 20 v / v) set flow of 1,0 ml/minute and discharge. Flux a solution of H2O : Acetonitrile or Methanol (30 : 70 v / v) for 30 minutes discharge it. It is recommended to perform the washing of Analytical Column at the end of each analytical run. Store the column in a solution of H2O : Acetonitrile or Methanol (30 : 70 v / v).

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  • HPLC COLUMN PROTECTION
    To save the analytical column Reverse Phase ZORBAX Eclipse Plus C18 (100 x 4,6 mm, 3,5 μ), the use of Javelin Colfilters (1 x 10 pcs.) cod. S90199511 is obligatory.
    HPLC COLUMN CONDITIONING
    Install a new analytical C18 Reversed-Phase column ZORBAX Eclipse Plus C18 (100 x 4,6 mm, 3,5 μ) .
    Disconnect the detector and filter 30 ml of H2O : Acetonitrile (20 : 80 v / v) set flow of 1,0 ml/minute. Don’t recycle the washing solutions. Flux 30 ml of a solution of H2O : Acetonitrile (80 : 20 v / v) at flow of 1,0 ml/minute. Don’t recycle the washing solutions. Condition the column with the mobile phase at a flow of 1,0 ml/minute and discharge the first 20 ml. Finally inject the Chemical Standard and verify the quality of the HPLC run.
    It’s NOT possible to make analysis at recycling phase. If room temperature is  20 °C store the Mobile Phase at 2-8 °C between an analytical session and another.
    COLUMN CLEANING
    Disconnect the detector. Flux 15 ml of H2O : Acetonitrile or Methanol (80 : 20 v / v) set flow of 1,0 ml/minute and discharge. Flux a solution of H2O : Acetonitrile or Methanol (30 : 70 v / v) for 30 minutes discharge it. It is recommended to perform the washing of Analytical Column at the end of each analytical run. Store the column in a solution of H2O : Acetonitrile or Methanol (30 : 70 v / v).

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