Hi! I have a gradient method using aqueous and acetonitrile phases and I use Agilent 1260 infinity I system. I need to transfer this method to Agilent 1260 Infinity II prime, but a had problems. using 1260, I had great peak shapes of all ones. But when I used 1260 prime system I had different retention times (something like 40 min on 1260 system and 45 min on 1260 prime system, for one of them).
Besides that uncharacterized peaks was obtained. Fronting peaks was obtained and it is a validated method using Agilent 1260. Now I have no time to change the original method. I just need a similar performance using agilent 1260 prime. Someone can help me?