Rinsing HPLC system



We mostly rinse the column and the system after the analysis with 70% acetontril in water.

So this will be in the HPLC system.

My question is if it doesn't matter when we then use a mobile phase with fosfate buffer or do we first have to rinx=se the HPLC system with a more aqueous solvent such as 50% methanol in water.




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