What is the cause of a shift in the FLD signal during a run?

We often run amino acid analysis with a HPLC FLD (G1321A). But recently we have some problems with our chromatograms.

For the detection of primary and secondary amino acids, a different excitation and emission wavelengt is needed. So for the analysis a timetable for the FLD signal is set up:

Recently we have noticed that, at 17,40 minutes, when the FLD switches its signal, it also causes a shift of the baseline in our chromatogram. Which means we are not able anymore to detect our secondary amino acids.

Does anybody has expercience with such a problem?

Thank you!

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0 sschombu over 4 years ago

Was that drop also present on previous runs? I don't think so. As the baseline seems to follow the changes in the gradient, I suspect more an application issue, rather than a real hardware issue, but I would rule that out by running the tests. Still the flap top peak needs some attention. Also remember that well degassed, fresh solvents of very good quality are vital for FLD operation.

Have a word with an application chemist. I'm looking at it from a hardware side.
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0 sschombu over 4 years ago

Was that drop also present on previous runs? I don't think so. As the baseline seems to follow the changes in the gradient, I suspect more an application issue, rather than a real hardware issue, but I would rule that out by running the tests. Still the flap top peak needs some attention. Also remember that well degassed, fresh solvents of very good quality are vital for FLD operation.

Have a word with an application chemist. I'm looking at it from a hardware side.
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