Hi Everyone. I ran GFS on Agilent 1100 HPLC, and the profile is atypical. It seems to be missing a peak and there is also a negative peak. Does anyone know what might be going on?
Mobile phase: 25 mM Ammonium Acetate, pH 8 (not filtered)
Flow rate: 0.3 mL/min
Wavelength: 280 nm
Column: AdvanceBio SEC 130Å, 7.8 x 300 mm, 2.7 µm (brand new)
GFS preparation: Reconstituted 1 vial of Bio-Rad GFS with 1 mL of HPLC Grade water, centrifuged, and injected supernatant at 5 uL.