GPC/MDS Triple detection and absolute molecular weight determination

Hello.

I use GPC/MDS Agilent 1260 Infinity II (RI, Visco, dual angle LS) and Agilent GPC/SEC Software version 2.2. Solvent is THF. Could anyone help me with absolute molecular weight determination, please? I did the LS calibration.

Do I have to fill dn/dc = 0,185 only?

Here I eliminate the points not aligned. Do I have to consider Mark-Houwink constants (K, alpha) from literature (polymer-solvent-temperature) in pairs? What I have to consider to get accurate conformation, Rg, Rh...?

Thank you very much for any information.

Kind regards

Andreja

Top Replies

andwoods 20 days ago +1

Hello Andreja, I just wanted to advise that 'data fit limits', have not been set correctly - please see the correct example below:

0 andwoods 20 days ago

Hello Andreja,

I just wanted to advise that 'data fit limits', have not been set correctly - please see the correct example below:
Cancel
Vote Up +1 Vote Down

Sign in to reply

Verify Answer

Cancel
0 RedRidingHood 3 days ago in reply to andwoods

Hello.

Thank you very much for your answer.

I am now sending the data processing of one sample from start to finish. I hope it's correct. I am interested in what is the criterion for determining the limits (third picture). Do I have to take into account a pair of Mark-Houwink constants from the literature? Can the relative and absolute molecular weights be very different? I will be very grateful for any feedback.

Best regards
Cancel
Vote Up 0 Vote Down

Sign in to reply

Verify Answer

Cancel

Was this helpful?