We are working to quantify the metabolites acetone, butanol, and ethanol from a bacterial cell culture using headspace analysis with manual injection. Our instrumentation includes the Agilent 5975 Series MSD and 8890 GC.
To create standard curves for each metabolite, we have run pure samples of butanol at various dilutions (1x, 10x, 40x, 100x, and 200x). We are using MassHunter Quantitative Analysis software for analysis, and we also have access to MassHunter Qualitative Analysis (12.0).
Could someone explain how to combine the data from all these dilutions into a single calibration graph for butanol? Additionally, we plan to make separate calibration graphs for acetone and ethanol. Once the calibration graphs are ready, how do we use them to determine the concentrations of these metabolites in an unknown sample?
We're relatively new to MassHunter, so detailed step-by-step instructions would be greatly appreciated. Thank you!
