Qualifier ratios reading high

Qualifier ratios jumped ten fold from one run to the next. What could cause this, auto tune was within spec. Previous run was fine.

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  • Jessica, it is very hard to make guesses or give recommendations without more information. 10 fold increase in ...what?  The qualifier ratio of what ions to what ions? 10 fold increase in peak area or height?

    If the mass spectrometer's tune was good before and good after these runs, it is probably not the MS.  Most instant large increases and decreases in peaks are injection issues like a plugged syringe or an autosampler having problems injecting the same amount.  

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