I run a method that requires 30 ng of bromofluorobenzene (bfb) on column to meet the method's tune check spectral evaluation. I have been preparing IS/SS secondary standards from stock standards according to calculations from a former operator of the system. I notice the calculations do not include anything for split ratio. I use an ATOMX purge and trap with the IS/SS prepared standard being autoinjected into the water sample. Once the x uL of IS/SS mix is injected into x mL of sample, in order to achieve the desired ng of bfb on column, should I be dividing this number by the split ratio?
Thank You.
