no signals found after tune

I have a problem with my GC-MS.
I was doing a run with HS-SPME-MS and when I did the next injection there was no signal. I repeated the procedure and injected the sample again with no signal. I did a tune to verify that everything was fine and the message "no peaks found" came out and the tune did not finish. The tune that was made previously was perfect.
Any idea what it could be?

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