I'm not sure if this is right place to pose questions of this nature but here it goes.
When trying to obtain the mass spectrum from a smaller peak on the slope of the matrix peak what is the best approach? How much does subtracting the matrix peak from the rider peak impact the accuracy of the mass spectrum of the rider peak? I attached an example TIC from my current analysis. My thought is that some mass fragment ions may be inaccurately represented in the rider peak due to the matrix peak. Is it better to subtract from baseline outside of the matrix peak? Thanks in advance.