Hi Community, I'm currently developing a HS GCMS method for determination of volatile extractables in 50:50 EtOH:Water extract and due to the high amount of EtOH in the extract the first minutes of the chromatogram is taken by EtOH peak (very broad peak) and in addition to that the EtOH is compromising the extraction of the most volatiles compounds in the headspace. My aim is to reduce the most out of EtOH going to vapor phase in the headspace. Current the incubation temp of the temp is 70C, shall I reduce the incubation temp? What else can I do to achieve that?
Many thanks in advance for any help you can provide on this matter.