Column flow/pressure drops during desorb

Hi everyone 

I am new in this platform and i am hoping to learn more. 

I recently removed my column and trimed it but when i replaced it back i have experienced the following: immediately after purge and trap desorbs the column flow drops for the set time of desorption. The tune report has passed. I have change the liner, gold seal, and septum but doesn't help the situation. 

I have been doing this same exercise(trimming) before when i was still using the chemstation software. Now the software is changed to mas hunter and since then we have been experiencing problems. Part of the other problem is that the pulsed plit injection doesnt work anymore. 

Could this dropping of column flow be connected to the software upgrade or it is a hardware problem? And what could it be? 

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