Column flow/pressure drops during desorb

Hi everyone 

I am new in this platform and i am hoping to learn more. 

I recently removed my column and trimed it but when i replaced it back i have experienced the following: immediately after purge and trap desorbs the column flow drops for the set time of desorption. The tune report has passed. I have change the liner, gold seal, and septum but doesn't help the situation. 

I have been doing this same exercise(trimming) before when i was still using the chemstation software. Now the software is changed to mas hunter and since then we have been experiencing problems. Part of the other problem is that the pulsed plit injection doesnt work anymore. 

Could this dropping of column flow be connected to the software upgrade or it is a hardware problem? And what could it be? 

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  • Can you share some screen captures from MassHunter Acquisition with your run method loaded of the  Inlets SSL-Front (or whichever one your P&T is connected to), GC Columns window, the Configuration/Columns window, and the Configuration/Modules window?   It could also be an issue inside the P&T. During desorb a valve inside the P&T puts the trap inline with the flow to the inlet while the trap heats up to desorb your compounds. If the valve is not working right, the trap is plugged or leaking, or a few other possibilities, the pressure/flow to the GC inlet won't be present.  

    Does the GC work fine without running the P&T?  If you just setup and start a run by pressing the start button, does it run?  

  • Please see the attached. I however as a routine maintenance of P&T rinse the sample pathway. After that i baked the system. Could i have done something wrong amd if yes how could i rectify. 

  • I have put a beaker with methanol at the end of the transfer line from the P&T and manually desorbs and i could see bubbles in the beaker. Couldn't this mean the p&T desorbs correctly? 

  • I'm in the US and this is the first time I've seen someone using kPa instead of psi.  The parameters look fine. I would recommend a longer Oven Equilibration time, like 1 minute or more.  Maybe increase your Scan threshold from 57, but I'd have to see the data file to know how high to go. The TIC background is not a huge number compared to some, but could be reduced by increasing the threshold.  You could certainly turn off acquiring and looking at the GC detector test signals.  

    Atune.u with tune EM of 2094. That's still usable, for sure. I'd look at the EM age, how long it's been since cleaning, maybe remedial cleaning, but no worries for the moment.

    MIB and Geosmin...I messed with those when I was an intern in like 1980 or so!

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  • I'm in the US and this is the first time I've seen someone using kPa instead of psi.  The parameters look fine. I would recommend a longer Oven Equilibration time, like 1 minute or more.  Maybe increase your Scan threshold from 57, but I'd have to see the data file to know how high to go. The TIC background is not a huge number compared to some, but could be reduced by increasing the threshold.  You could certainly turn off acquiring and looking at the GC detector test signals.  

    Atune.u with tune EM of 2094. That's still usable, for sure. I'd look at the EM age, how long it's been since cleaning, maybe remedial cleaning, but no worries for the moment.

    MIB and Geosmin...I messed with those when I was an intern in like 1980 or so!

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