I notice that during MHE with four injection, each extraction contains same amount/intensity of a component (VA). No degradation behavior is observed. What could be a reason? How could we quantify in such case?

Hello Tahir 

I’m sorry for the delayed reply to your message.  Do you still need assistance with this? If you do, please take into consideration the notes below. However, if the issue was resolved, could you please share what the resolution was?  Thanks!

For being able to answer your questions we would need more details regarding sample composition and sample preparation. Alternatively, you can always contact Agilent for Application Support.