I am attempting to validate this method on a GC-ECD 6890 Series. This method has 3 analytes, when I run the standard that i have with all 3 analytes I only see peaks for 2 of them. I ordered the standards separately in order to identify the peaks and it is going to take a little while for them to come in. I set up the conditions exactly how the method stated it to do. Can anyone advise on what I may be doing wrong in order to not see all 3 analytes? I played with the method a little bit and made the run time longer and still no 3rd peak.
Thanks in advance.