Hello everyone!! I am new to this forum
I have been given an Agilent GC/FID 7890B and asked to set a fatty acid analysis method.
The thing is that I have been having problems with the base line recently and since I am new to GC chromatography I don't know what is causing it and how to solve it.
Column: HP-88 60 m x 0,25 mm, 0,2 µm ; Oven inicial temp: 125 ºC ; Oven max temp: 220 ºC ; Carrier gas: He ; Detector: FID 260 ºC ; Sample solvent: n-Hexane
If there is anything else important that I should mention let me know!
So, a month ago this is how a run of a 10 ppm 37-FAME sample would look like:
And this is how a 10 ppm 37-FAME sample looks like right now:
And if this is any helpful here is an image of a Blank sample on the same run as the previous sample:
I would appreciate any help or criticism !!