Reponse very high

Good morning -

 

The chromatography response on my instrument was normal and now it was really high. The 111120 file was my previous response where everything looked normal, and the tph057 file is what it looks like now. This is on an HP5890 and there is no indication of a leak at the detector. All method variables remain the same between both injections. Can anyone help me where to start?

 

Thanks!

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