My method details:
GC Conditions:
- Column: HP-5MS UI (30 x 0.32 x 1.00)
- Detector: FID (@ 260C)
- Carrier Gas: He (2 mL/min)
- Liner: 1mm straight inert (Restek)
- Inlet Temp: 230C
- Split: 10:1
- Oven Program: 35C, hold for 3 minutes, 15C/minute to 230C
Head Space Conditions:
Vial Size: 20mL (1mL of sample)
Diluent: DMSO
Oven Temp: 145C
Loop Temp: 155C
Transfer Line Temp: 170C
Vial Equilibration Time: 10 Minutes
Vial Pressure Time: 1 Minute
Loop Fill Time: 0.5 Minutes
Loop Equilibration Time: 0.2 Minutes
Sample Inject Time: 0.5 Minutes
I am seeing DMSO elute and drag off the column, and it never returns to baseline. I do not mind the solvent diluent having a poor peak shape since that is expected but because it is so broad I have seen that it carries over into subsequent injections (see attachment) resulting in a poor baseline. What could be causing this?
Thanks!