Tapered syringe needle increases abundance?

Why do my sample abundances increase X3 using a tapered 23/26 gauge syringe  vs a straight 23gauge syringe? I did watch to be sure it was delivering 1uL of sample. My values increased from just over 300 to over 1000 with the change in syringe.

 

According to this table, the internal diameter of the 2 gauges are the same.   Even if the internal diameter of the smaller bore were smaller, how would this increase the amount of sample introduced onto the column?  

 

 

Running on a 7890GC.

 

Syringe Part #'s

23/26gauge tapered Agilent 5181-1267

23 gauge straight Thermo 365D1576

Parents
  • Hello,

     

    From my perspective, the syringe length (depth of insertion), sample dispersal due to shape and flaws in the needle, how the sample hits the liner/glass wool surfaces.

     

    Are you seeing comparable RSD's from multiple injections, using both types of syringes?

     

    If everything else remained the same, the above properties may add some explanation of the increased efficiency of column loading.

Reply
  • Hello,

     

    From my perspective, the syringe length (depth of insertion), sample dispersal due to shape and flaws in the needle, how the sample hits the liner/glass wool surfaces.

     

    Are you seeing comparable RSD's from multiple injections, using both types of syringes?

     

    If everything else remained the same, the above properties may add some explanation of the increased efficiency of column loading.

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