HP-INNOWAX COLUMN

Question

Hello, I have a question about the capability of the column HP-INNOWAX can I inject a chloroform sample in it? I&rsquo;m trying to detect PHB in those samples

Dr. Know · Accepted Answer

Hi D.Tobias, 
 just to be clear you cannot analyze PHB with GC unless you use Pyrolysis-GC or, as Mariana already mentioned, you only analyze the monomer. 
 For the monomer determination you have to split the polymer into the monomers by acidolysis and then derivatize the acid with methanol to the methyl ester. This is often done in a single step: PHB in chloroform plus methanol plus sulfuric acid, 50 &deg;C for up to 2 days, removing the acid, dry the extract with sodium sulfate and then inject into a GC. A Wax column wouldn't be my first choice as apolar phases will work perfectly. 
 Please see these articles for the process: 
 
 https://doi.org/10.21769/BioProtoc.2748 
 https://doi.org/10.1016/j.egypro.2017.10.195 
 
 For pyrolysis: https://doi.org/10.1016/j.ijbiomac.2021.01.108 
 Oh, and to answer your first question chloroform can be injected in GC as long as there is no condensation in the phase. 
 Regards, 
 Norbert

HP-INNOWAX COLUMN

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