Scientists commonly use SEC to measure aggregation of proteins in solution. A size exclusion chromatography (SEC) column is a high-performance liquid chromatography (HPLC) column that separates analytes by size. The column is made of a cylinder-shaped housing that has been filled with porous particles, which serve as the stationary phase.
The size of the pores determines what range of analyte sizes can be separated by that column. Agilent recently published new AdvanceBio SEC Columns FAQs, which include:
- SEC and what can be separated by SEC
- SEC column tips and tricks, including what makes a good column, how to choose a column, column dimensions, installation, and maximizing column life
- How to choose mobile phase and the use of organic solvents in mobile phase
- Protein standards
- Concentration and injection volume
- Peak eluting
- Detector for SEC