How to measure Mitochondrial acidification from Glycolytic Rate Assay?

Hi,

I recently performed the Glycolytic Rate Assay and normalized the data using cell counts. I organized the data into two sets: before and after ROT/AA, and post 2-DG.

  1. How do I calculate the mitochondrial acidification rate from this data?
  2. I noticed that I only obtained ECAR values for my conditions, and for some of my conditions, I received PER values, but for others, the values were zero. Could you help me understand why this might be happening?

Thank you.

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