What solvent for respiration reagent on isolated mitochondria assays?

Question

Hi Courtney, 
 Thank you for your response. I have a question regarding the selection of the solvent for the respiration reagent. I've encountered conflicting information on this matter. The literature (PMID: 35771443) indicates the use of DMSO, whereas the information provided to me suggests a solution in 95% ethanol. Could you please advise me on which option to choose? 
 I eagerly await your response. 
 
 Best Regards, 
 LD

Courtney Watts · Accepted Answer

Hi LD, 
 
 You are very welcome! Our protocol recommends using 95% ethanol (do not use 100 % ethanol as it contains traces of benzene, which is detrimental to mitochondrial function) rather than DMSO. Also be sure not to add ethanol to the injection ports as this can cause leaks/injection issues in your assay. 
 
 Thank you and let me know if you have any other questions. 
 Courtney

What solvent for respiration reagent on isolated mitochondria assays?

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