Seahorse Xfe96 mito stress maximum OCR

Question

I recently ran a mito stress test using the Seahorse Xfe96 and my OCR (pmol/min/cells) values were higher than I typically see for published mitostress assays. 
 Basal OCR for control and treatments ranged from 150-280 and maximum OCR went up to 580. I am wondering if these values are too high to be accurately measured by the Xfe96 or if these values are acceptable? 
 Thank you in advance for any help! 
 Rebecca

Courtney Watts · Accepted Answer

Hi Rebecca, 
 
 You are very welcome! It is hard to say without seeing the data, unfortunately. We make the recommendation of 160pmol/min as the maximum because when it starts higher than that, once the FCCP is injected, the cells begin to work so hard that most/all of the O2 in the wells will be consumed, resulting in anoxic cells. If you would like to send me the data file, I can better advise you! 
 Thank you, 
 
 Courtney