seahorse problems

Hi, we aregenerating adipocytes from human pluripotent stem cells but we face some problems with the masure of the oxygen consumption because our cells do not grow in monolayers but in multiple layers. The thing is that when we apply the classical protocol to maesure oxygen consumption with the seahorse technology in our cells, they start to deatach. has anybody faced this kind of issue? If yes do you have any suggestion? Thanks  Stefania

Was this helpful?