• State Verified Answer
  • +1 person also asked this people also asked this
  • Replies 1 reply
  • Subscribers 6 subscribers
  • Views 1412 views
  • Users 0 members are here
Options
Related

QuikChange Lightning Site-Directed Mutagenesis Kit

J.Xiang

Is the “QuikChange Lightning Site-Directed Mutagenesis Kit” above the same component as part of the mutagenesis reagents in the QuikChange HT Protein Engineering System (Part Number:G5901B)? The name of some of the reagents and the reaction volume are similar so I wonder if we can adapt the “QuikChange Lightning Site-Directed Mutagenesis Kit” in our lab with our own mutation oligo library. Thanks!

Parents
  • +1

    While the names of the products and their reagents appear similar, there is a (proprietary) qualitative difference between them. As a result, the QuikChange Lightning product is more suited to the generation of a single mutation within a targeted sequence using a particular pair of primers. Alternatively, the QuikChange HT product is more suited to the generation of a library of mutagenized sequences using a group of custom-designed primers. Consequently, in order to generate optimal results, it is not recommended that the reagents from one product be substituted within the protocol of the other. 

Reply
  • +1

    While the names of the products and their reagents appear similar, there is a (proprietary) qualitative difference between them. As a result, the QuikChange Lightning product is more suited to the generation of a single mutation within a targeted sequence using a particular pair of primers. Alternatively, the QuikChange HT product is more suited to the generation of a library of mutagenized sequences using a group of custom-designed primers. Consequently, in order to generate optimal results, it is not recommended that the reagents from one product be substituted within the protocol of the other. 

Children
No Data
Was this helpful?
Privacy Statement
Terms of Use
Contact Us
Site Help