Our New AriaMx realtime PCR machine, one plate need to run two times

We just buy a AriaMx realtime machine.  We run the b-actin with SYBR 2X mixture we used before in a ABI machine. The realtime RT-PCR using the protocol 95C 2min, 95C 5seconds and 60C 30 seconds. No signal in the first time run, no touch the machine, just re-run the plate, the b-actin signals melting curves as good as our previous machine.  We confused the problem, the ABI SYBR 2Xmixture not match this machine ?? anyone has experience to resolve the problem. Thanks a lot !!

  • Hello,

    There are a couple of different possibilities for why you did not see amplification in the initial run, but were able to see the melting curve afterwards. To learn what the real cause was, it will be necessary to see the original data files from the instrument. To help solve this problem, please send the files to qpcr@agilent.com

    Thank you.

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