AssayMAP Bravo Protein Sample Preparation Platform — Resources

Created by mvandamm 3 months ago | Last modified by kristen.sensabaugh 3 months ago

Support and educational resources for the AssayMAP Bravo.

Product Pages

AssayMAP Bravo Protein Sample Preparation Platform
- Includes an overview of the AssayMAP system, literature, videos, and more
AssayMAP cartridges
- With information about types of cartridges available and resin chemistry details

Technology Explained

AssayMAP Bravo Fundamentals webpage
- What AssayMAP Bravo Platform is and how it works
- FAQs to learn what the system can do
Automated protein sample preparation that is easy to use
- Animated overview of the benefits of the AssayMAP Bravo Platform (1:42 min)
Agilent AssayMAP BRAVO: For Protein and Peptide Sample Prep-subtitles
- Animated overview of the AssayMAP in motion (5:20 min)
Unravelling the Myths of Lab Automation
- Video addressing common misperceptions about lab automation (3:13 min)

Webinars and Videos

Highlights:

Automated Protein and Peptide Sample Prep in Regulated and Nonregulated Labs Just Got Easier
- Steve Murphy, Ph.D. from Agilent Technology (48:19 min)
- Overview of the AssayMAP Bravo and the capabilities of the AssayMAP Workbench 4.0
An Automated and Faster Affinity Capture Method for Biotransformation Assessment on Site Specific Antibody Drug Conjugates
- Aarti Jashnani, M.S. from BMS (20:18 min)
Automated assay for MHC-bound peptide purification using AssayMAP
- Lichao Zhang, Ph.D. from Chan Zuckerberg Biohub (23:06 min)
- Automating Immunopeptidomics sample prep with the AssayMAP Bravo
Automating MRM-Based Protein Quantification
- Tasso Miliotis, Ph.D. form AstraZeneca (46:59 min)
Peeking Under the Hood of Cellular Signal Machinery with Targeted Proteomics Assays
- Jason Russell, Ph.D. from Agilent and Jacob Jaffe, Ph.D. from Broad Institute (57:54 min)
Use of AssayMAP for Proteomics and Biologics Developability
- Presented by Mahmud Hossain, Ph.D. from Sanofi (21:21 min)
See all AssayMAP webinars/videos

Publications

AssayMAP citation index is a complete list of peer-reviewed publications (<300) and app notes (<40).
- Searchable key words summarized on page 2
Coming soon - Focused AssayMAP Citation Indexes are derived from the AssayMAP citation index but are focused on specific areas of interest for ease of use.
- Antibody Drug Conjugates
- Fractionation
- Immunocapture
- Immunopeptidomics
- Pharmacokinetics
- Phosphoproteomics
- Protein-Protein interactions

Application Notes

Highlights (complete list can be found in the AssayMAP citation index):

Automated MHC-Associated Peptide Enrichment for Immunopeptidomics Analysis Using Agilent AssayMAP Bravo Large Capacity Cartridges (English, Chinese)

Introducing Semi-Automated GC/Q-TOF Screening with the AssayMAP Bravo Sample Prep Platform for Routine Antidoping Control (English, Chinese, Japanese, Korean)

A streamlined drug-to-antibody ratio determination workflow for intact and deglycosylated antibody-drug conjugates (English, Japanese)
An Integrated Workflow for Intact and Subunits of Monoclonal Antibody Accurate Mass Measurements (English, Chinese, Japanese)
Agilent AssayMAP Bravo Technology Enables Reproducible Automated Phosphopeptide Enrichment from Complex Mixtures Using High‑Capacity Fe(III)‑NTA Cartridges (English)
Host Cell Protein Analysis Using Agilent AssayMAP Bravo and 6545XT AdvanceBio LC/Q-TOF (English, Chinese, Japanese)
Determination of Drug-to-Antibody Ratio for Antibody-Drug Conjugates Purified from Serum using Automated Affinity Purification, LC/MS Analysis, and Novel DAR Calculation Software (English, Chinese, Korean)
Rapid Antibody Digestion Enabled by Automated Reversed-Phase Desalting on the Agilent AssayMAP Bravo Platform (English)
Automation of Sample Preparation for Accurate and Scalable Quantification and Characterization of Biotherapeutic Proteins Using the Agilent AssayMAP Bravo Platform (English)
Automation for LC/MS Sample Preparation: High Throughput In-Solution Digestion and Peptide Cleanup Enabled by the Agilent AssayMAP Bravo Platform (English, Chinese)
Workflow Automation for LC/MS: In-Solution Protein Digestion, Peptide Cleanup, and Strong Cation-Exchange Fractionation of Peptides Enabled by AssayMAP Technology (English, Chinese)

Posters

Highlights:

Peptide Mapping of Tryptic Digests for mAbs using a novel ECD cell on the 6545XT AdvancedBio LC/Q-TOF Mass Spectrometer (English)
Analysis of Host Cell Protein Using Automatic Offline Fractionation and LC-MS/MS (English)
Characterization of Automated Sample Preparation Workflows Featuring Phosphopeptide Enrichment using TiO2 Microchromatography Cartridges on a Precision Liquid Handler