Hello,
I am using the tapestation to analyze a PCR product. When using the region tool, I have extracted between 250 and 1000, as well as between 250 and 5000, but I am getting concentrations, average sizes and region molarities that do not match up.
Example for one sample:
| From [bp] | To [bp] | Average Size [bp] | Conc. [ng/ul] | Region Molarity [nmol/l] | % of Total |
| 250 | 5000 | 814 | 44.8 | 133 | 93.15 |
| 250 | 1000 | 468 | 36.7 | 127 | 76.46 |
These numbers look internally consistent (concentration slightly increases, molarity as well) But if you were to calculate the molarity yourself using the average size and given concentration, however, there is a discrepancy for the largest extracted region.
| Average Size [bp] | Mass weight (dsDNA) | Conc. [ng/ul] | Conc (nmol/l) |
| 814 | 501411.2 | 44.8 | 89.3478246995679 |
| 468 | 288295.96 | 36.7 | 127.299737394863 |
I don't understand where this discrepancy comes from. I am concerned that the average size is not being calculated correctly, which is the thing I am most interested in here, so that would be troublesome.
Thanks in advance!