Can we run uncut plasmid DNA using the the bioanalyzer chips?

We tried to run uncut and cutted plasmid DNA using the High sensitivity DNA chip using Bioanalyzer 2100.  The uncut plasmids showed peaks as expected, BUT we were not able to see anything on the uncut plasmid, like it was empty. We run agarose gels from the same samples, so the uncut plasmid was there, for sure. We repeated the bioanalyzer runs and the results were exactly the same. My question is, can we actually succesfully run uncut plasmid DNA using any of the Bioanalyzer chips? Or there is a special sample preparation that has to be done for this particular purpose? If we can't, What is the issue? It has anything to do with the supercoil formation? 

We are looking forward for your input, since we were considering the use of the bioanalyzer for plasmid quality control.  Please, advice! Thanks! 

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  • Hi eamaral,

     

    Unfortunately, there is no Bioanalyzer section on the community at this point in time. Your best course of action at this point in time would be to contact the Agilent Bioanalyzer support team. You can reach them by emailingbioanalyzer@agilent.com .

     

    NOTE: This support email address is for customers in North America. If you are from another region, then please use the following link to determine the appropriate Agilent contact information: Contact Us | Agilent

     

    Thanks,
    Matt

Reply
  • Hi eamaral,

     

    Unfortunately, there is no Bioanalyzer section on the community at this point in time. Your best course of action at this point in time would be to contact the Agilent Bioanalyzer support team. You can reach them by emailingbioanalyzer@agilent.com .

     

    NOTE: This support email address is for customers in North America. If you are from another region, then please use the following link to determine the appropriate Agilent contact information: Contact Us | Agilent

     

    Thanks,
    Matt

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