1. Can modification of amino acid (or base) influence the result (migrate time and/or molarity) of Agilent 2100?
2. Using DNA 1000 kit, I found Agilent 2100 can detect the ssDNA, but with a wrong size (or concentration, not sure yet). About the wrong size peak, could it be any different if I increase the ssDNA input or use a modified ssDNA (5'-phosphorylation e.g.)?
3.What about the Y-adaptor used in NGS? the migrate time is faster or slower than dsDNA?