We are using the tapestation to validate the annealing of oligos. Our duplex-oligo is made of a 30 nucleotides oligo and a 15 nuleotides oligo. It is therefore partly dsDNA and ssDNA.
Recently we have had different results with a new order of oligos. to validate them, we have run them both together in the tapestation. One migrate around 50bp, the other migrates around 25bp. I understand this is the low end of the size detection, but the difference in size is very clear, as you can see, below.
My question is how is this oligo expected to migrate? Any experience of doing this? any theoretical idea?
Many Thanks for your answer,