Optical signal too high

Hi Claudie and Nikki, That error comes up when the 2100 Bioanalyzer optics detects a very high fluorescence signal. Typically, this can happen due to sample overloading and/or if chip gets prepared using cold reagents. Please indicate what assay are you running, and whether the error is coming up irrespective of the assay type being run. To be sure we provide you with most accurate analysis, please share a recent example file ending with .xad at electrophoresis@agilent.com with attention to Shweta. Thank you, Shweta

Hi Claudie and Nikki, That error comes up when the 2100 Bioanalyzer optics detects a very high fluorescence signal. Typically, this can happen due to sample overloading and/or if chip gets prepared using cold reagents. Please indicate what assay are you running, and whether the error is coming up irrespective of the assay type being run. To be sure we provide you with most accurate analysis, please share a recent example file ending with .xad at electrophoresis@agilent.com with attention to Shweta. Thank you, Shweta

Thank you  so much Shweta, 

I think it was the cold reagents.

Claudia