Bioanalyzer-Agilent Protein 230

Question

Hello,

I’ve been using the Agilent Protein 230 kit to run my protein samples on the bioanalyzer. What could cause some bands to appear more “crisp” while others appear more blurred? Could it be species very close to each other that are hard to distinguish?

Lanes 3,4,7,8 are digested polyclonal antibodies.
Lanes 9,10 are not digested polyclonal antibodies .
Lanes 2 and 6 are digested monoclonal antibodies.

I have attached an image as an example from one of my recent assays. I can provide you with more information regarding the experiment if needed.

Thank you,

Joanna

Ian.J · Accepted Answer

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