How to clean SPS 4 probe?

I found major deposits within my autosampler probe after doing an analysis with MP-AES which the rinse solution (high purity water) wasn't able to remove.

How do I remove these deposits within both the probe and the autosampler tubing?

Thanks!

  • Hi Shane,

     

    What type of samples are you analysing and what is the matrix?

    I typically prefer to rinse with the same matrix as the samples being analysed. This helps to keep the tubing clean. Once a probe is visibly dirty it can be difficult to clean. I would suggest replacing the probe and using a more aggressive rinse. The minimum i suggest is 2% HNO3, although if you are measuring something basic, a different rinse may be more appropriate.

    If you want to try to clean the probe running some acid through it may be able to clean it. 10% HNO3 may do what you need. You could use a syringe to connect to the probe to rinse it, or use the autosampler pump. 

    Regards,

    Peter

  • Hi Shane,

    We have a recommended regeneration procedure for the probe, usually used for ICP-MS to get rid of sticky elements that contribute to the background.  Note this is an aggressive strip and clean that you can only perform about 3-times before you will need to replace the probe.

     

    4-step rinse by pumping ~50mL of:

    1. Ammonium Hydroxide 1:1 dilution with Ultrapure water
    2. conc Nitric acid 1:1 dilution with Ultrapure water
    3. conc HCl 1:1 dilution with Ultrapure water
    4. Ultrapure water

    Aim to pump 50ml in 30-40 min each (@1.5mL/min)

     

    As Peter mentions suggest relook at your rinse to ensure it is cleaning out your sample matrix sufficiently each time.

     

    Hope that helps.

     

    Rgds, Gareth

  • Hi everyone, 

     

    Thank you Peter Riles and Gareth Pearson for the brilliant feedback. Prior to your replies managed to clean the probe effectively by using a very dilute detergent (Hellmanex III) in an ultrasonic batch. 

     

    The following method was used:

    1. Draw water through the probe into the tubing connected to the probe using a syringe.

    2. Submerge the tubing and the probe together into the ultrasonic bath.

    3. Ultrasonicate for 15 minutes. 

     

    Following this I noticed that the water alone was sufficient to thoroughly clean the probe itself however a small blockage was still observed in the tubing.

    4. Add a very small aliquot of detergent into the ultrasonic bath.

    5. Using a syringe again, draw the dilute detergent solution into the probe and tubing and submerge fully in the bath.

    6. Ultrasonicate for a further 15 minutes.


    No deposits remained on/in the probe or tubing.

     

    I then used Peter Riles advice of using the nitric acid (2%) as a rinse solution and this has functioned well at reducing deposit formation. If the need should arise in the future for an aggressive rinse according to Gareth Pearson's contribution, I will keep the method in mind.


    Thank you all!

     

    Regards,

    Shane 

  • Thanks for sharing your experience Shane so we can also learn from you - glad to hear you have had success!

     

    Rgds, Gareth

  • This could be work for a sample probe of Headspace Autosampler? 

  • This question has been marked as assumed answered.

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