what can we do to improve internal standard counts consistency? since sometimes RSD% for internal standard counts were beyond 5%?

Hi, 

. 1. recently, we changed the tubing for internal standard and sometimes found, RSD% for internal standard counts was beyond 5% which raises red flags ( see the below picture) 

2. These warnings showed once after 6 or 9 injections.  we noticed some very small bubbles were created and accumulated when the sampler was rinsing. 

3. what should we do to make internal standard reading consistent? 

Thank you

Best regards, 

DI Xu 

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