Does the tune start off looking okay, but then fails?
If so, it might be a corrupted tune file.
Yes it does start off looking normal. However, all the tune files give this error.
I forgot another thing. One of the filaments previously gave the error "Tune action stopped: Divide by zero!", but now both are giving the error in the first post
Try going into manual tune, don't change any parameters then physically go to file then save tune. If this doesn't work you can find default tune files or even previous tunes in the directory:
C:\MassHunter\GCMS\1\5975 then the sub folder for previous tunes.
C:\msdchem\1\5975 then sub folder for previous tunes.
Load a previous tune for maybe a few months ago then save as the current tune file used (atune, etune etc) then try tuning again. it will take serveal tunes to get optimal but as sullivana mentioned it could be the tune file is corrupted.