• Created : 
  • Modified : 
  • Category :  Agilent Knowledge Portal
  • Entry Type :  Article
  • Product Type :  Real-Time PCR (qPCR)
  • Component :  qPCR Systems
  • Product Name :  AriaMx Real-time PCR System
  • Task :  Troubleshooting
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Performing Motor Calibration on the AriaMx

Answer

This Information Applies to: Agilent AriaMx real-time PCR system

Issue

Motor calibration (MC) is a procedure that identifies the location of each well scanned by the optical modules for each slot.

Background

This calibration is completed before the instrument is shipped, so you usually do not need to do it again. However, if the instrument’s touchscreen shows a notification that motor calibration is required (as shown in Figure 1), click OK and follow the on-screen instructions.

 

Figure 1. AriaMx raw data plots.
 1. Notification when motor position is not calibrated

If Agilent technical support asks you to perform a motor calibration, please perform the following procedure.

 

  
Tip: Adding and removing optical modules does not cause the instrument to require a motor calibration.

 

Items required

  • FAM optical scanning module
  • A 96-well plate and caps or film identical to the consumables used in the AriaMx
  • 20 µL of water for each well

Steps to Follow

  1. Access Settings:

    • On the AriaMx touch screen, select to Settings>System Settings>Motor Calibration (Figure 2).



      Figure 2. AriaMx touch screen system settings.

  2. Initiate Motor Calibration:

    • A list of all installed filters will appear.
    • Click Calibrate on the Motor Calibration screen (Figure 3).

      g

      Figure 3. Motor Calibration screen

  3. Prepare the plate:

    • Insert a capped plate filled with 20 µL of water or background buffer in all wells
    • Place the plate into the AriaMx instrument.
    • Click OK.

  4. Configure Optical Modules:

    • Remove all optical modules except for the FAM module.
    • Insert the FAM module into slot 1 and click OK.

  5. Follow on-screen instructions:

    • Follow the on-screen instructions to move the FAM module into all slots in turn to complete the calibration.

  6. Perform background Calibration:

    • After the motor calibration is complete, an error bubble will appear instructing you to perform background calibration.
    • Re-insert all optical modules into their desired locations and perform the background calibration.
  
Tip: After performing the motor calibration, it is crucial to verify the motor calibration values using the AriaMx support tool. Before starting the motor calibration, download our AriaMx Support Tool software here. You can find here the Aria Support Tool User Guide. Once you have run the motor calibration, verify the motor calibration values by following the protocol at page 9 of the Aria Support Tool User Guide.

 

   Tip: View Performing a Background Calibration on the AriaMx

 

 
earn how to effectively operate your Agilent AriaMx:
AriaMx Real-Time PCR System Setup and User Guide on Agilent.com
Aria Real-Time PCR Software User Manual on Agilent.com

For Research Use Only. Not for use in diagnostic procedures.

PR7001-3605

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