This Information Applies To: AriaMx Real-time PCR System Issue The saturation level is unique for each optical module after the offset of calibration factor and background calibration. The FAM, ROX, HEX, CY3 & CY5 optical modules will saturate at approximately 32,000 RFU while ATTO optical module will saturate about half as high, at approximately 16,000 RFU. Background A fluorescence signal that ends in a horizontal line at the top indicate that the signal has reached saturation levels...

This Information Applies To: Agilent AriaMx real-time PCR system Issue Scratches on AriaMx optical modules can lead to a reduction in their performance. Background Scratches on an AriaMx optical module are shown in Figure 1 . Figure 1 . Optical module with horizontal scratches Scratches on the AriaMx optical modules can have several effects, including: Reduced optical clarity: scratches can obstruct the path of light, affecting the quality of imaging and data acquisition. Decreased...

This Information Applies To: Agilent AriaMx real-time PCR system Issue Storing the Agilent AriaMx plasticware to ensure its quality and performance. Background AriaMx plasticware is made of polypropylene, a durable and flexible material that is widely used in biology laboratories. Polypropylene has several advantages, such as: Resistance to chemicals, acids, and bases, making it suitable for various PCR applications. A low binding affinity for DNA, RNA, and proteins, minimizing...

This Information Applies To: Agilent EI Source Issue: During normal operation of the GC/MS, the EI SST/Inert ion source will need to be disassembled to proceed with abrasive ion source cleaning and / or routine replacement of consumable parts (filaments and ceramic insulators). The ion source will then need to be assembled prior to re-installing. Correct abrasive cleaning, assembly, and installation are essential to robust and reliable operation. Tools Needed: • 597X Tool Kit and Cleaning...

This Information Applies To: Agilent AriaMx real-time PCR system Issue When running the AriaMx with tube strips, and a tube strip is not present in every well, fluorescence is detected in the wells without plasticware. Background When using the AriaMx, it is expected behaviour that the instrument may detect fluorescence in wells that don't contain plasticware. The AriaMx scans all 96 wells with all optical modules present in the instrument. Fluorescence can reflect off the thermal block...