This Information Applies To: Agilent OpenLab CDS (Rev. 2.x)
This article outlines the step-by-step instruction on how to extract a UV spectrum via right click.
If your data contains UV spectral data, you can extract spectra from your chromatogram. You can either:
- Extract a UV spectrum via a right click.
- Extract a UV spectrum or multiple spectra using the spectrum extraction tool.
- Acquisition method in DAD is set to store All spectra see Figure 1. You can read more about parameter settings to store spectra here.
Figure 1: Store All Spectra setting in acquisition method for DAD
- The injection is linked to a processing method that supports manual UV spectra extraction 3D UV Quantitative (Figure 2) or LC/MS Sample Purity (Figure 3).
Figure 2: 3D UV Quantitative Processing Method
- To be able to carry out the procedure as described, the user needs the privileges Data Processing > Do manual spectrum extraction; Processing Method > Edit spectrum extraction parameters and Processing Method > Edit spectra parameters. Privileges are configured in the Control Panel.
Steps to Follow
- (Optional) Extract UV background spectrum (see article How to Extract a UV Background Spectrum in OpenLab CDS)
- In the chromatogram, right-click the peak, then in the context menu, click Extract spectrum.
The extracted spectrum is shown in the UV Spectrum window.
The time of the peak apex as well as the time used for background subtraction are shown in the spectrum name.
The extracted spectrum is listed under the details for the individual injection in the injection tree. Figure 4 shows an example of how to extract a UV spectrum via a right click (with UV background subtraction).
You can also extract UV spectra using the spectrum extraction tool. See articles How to Extract a UV Spectrum with the Spectrum Extraction Tool in OpenLab CDS and How to Extract Multiple UV Spectra in One Step in OpenLab 2.x.
Learn how to effectively operate your Agilent OpenLab CDS Software:
HPLC-OLII-1121e - Agilent Infinity LC Systems with OpenLab CDS – Acquisition Method e-learning course available from Agilent Education