This overview offers a look at gradients and how they are developed. We will also discuss common pitfalls and how to address them.
- Why Use Gradients?
- The Gradient Equation
- Gradient Steepness
- Mobile Phase Modifiers and Drifting Baselines
- Dwell Volumes
- Effect of Dwell Volume on Ruggedness–Gradient Separations
- Autosampler Programming to Assist with Gradient Optimization