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  • Category :  Agilent Knowledge Portal
  • Entry Type :  Article
  • Product Type :  Analytical Software Suite
  • Component :  OpenLab Chromatography Data System
  • Product Name :  OpenLab CDS OpenLab ChemStation
  • Task :  Data Analysis Operation
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Calculating Area% and Height% in OpenLab Software

Answer

This Information Applies To: Agilent OpenLab CDS , Agilent OpenLab ChemStation

Issue

Calculation of Area percent or Height percent for uncalibrated compounds in OpenLab software. If all components respond equally in the detector, then Area% and Height% provides a suitable approximation of the relative amounts of components.

Resolution 

  • Area Percent Calculation:
    • The Area% calculation procedure reports the area of each peak in the signal as a percentage of the total area of all peaks in the signal.
    • Area% does not require prior calibration and does not depend upon the amount of sample injected within the limits of the detector. No response factors are used. If all components respond equally in the detector, then Area% provides a suitable approximation of the relative amounts of components.
    • Area% is used routinely where qualitative results are of interest and to produce information to create the compound table required for other calibration procedures.
    • The Area% report is suitable for analyses in which the difference in detector response of the components is not significant.

  • Height Percent Calculation:
    • The Height% calculation procedure reports the height of each peak in the signal as a percentage of the total height of all peaks in the signal.

Correction factors are not applied in Area% or Height% calculation.

Area% and Height% are shown as default under Injection Results in OpenLab CDS (Figure 1). 

         

Figure 1: Area % and Height % reported under Injection Results 

Area% and Height% are reported in Single Injection Report in OpenLab CDS (Figure 2). 

               

Figure 2: Area % and Height % reported in Single Injection Report

Area% is shown as default under Integration Event Table in OpenLab ChemStation (Figure 3).


Figure 3: Area% reported under Integration Event Table

Area% is reported in the Short_Area report template. However, height% needs to be added to the report template in OpenLab ChemStation (Figure 4). 


Figure 4: Area % reported in Short_Area report 

 
Learn how to effectively operate your Agilent OpenLab Chromatography Data System:
Agilent 1260 Infinity LC Systems with OpenLab CDS e-learning course available from Agilent education.
Agilent 1260 Infinity LC Systems with OpenLab CDS ChemStation Edition e-learning course available from Agilent education.
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