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Automated Electrophoresis Articles Full Power Cycle for Agilent Parallel Capillary Electrophoresis Instruments
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      • Failed to Build Pressure Error on a Parallel Capillary Electrophoresis Instrument
      • Run Aborted and Target Z Position Error on Parallel Capillary Instruments
      • Analysis of Single Stranded DNA on the Fragment Analyzer
      • How to do a NaOH Flush on an Agilent Fragment Analyzer
      • Agilent Fragment Analyzer Message: Setup Incorrect - Ladder File does not Exist
      • Agilent Fragment Analyzer or Femto Pulse: Run Aborted, Error Moving Syringe Plunger
      • Agilent Parallel Electrophoresis Capillary Array Disposal
      • Re-ordering the Syringe for Cleaning the Reservoir Vent Valve of the Agilent Fragment Analyzer, Femto Pulse, or ZAG
      • DV200 Analysis of FFPE RNA Samples with the Fragment Analyzer
      • Agilent Capillary Array Installation on Parallel Capillary Electrophoresis Instruments
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      • How to Clean the Capillary Array Window on Agilent Parallel Capillary Electrophoresis Instruments
      • Reservoir Vent Valve Cleaning for Parallel Capillary Electrophoresis Instruments
      • Full Power Cycle for Agilent Parallel Capillary Electrophoresis Instruments
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      • How to Use Mineral Oil to Prevent Evaporation on Agilent Parallel Capillary Electrophoresis Systems
      • Running Dye Labeled Samples on Agilent Parallel Capillary Electrophoresis Instruments
      • Insert Disk Error in the Agilent Fragment Analyzer Controller Software
      • BAC Method Missing from the Agilent Femto Pulse Software

Full Power Cycle for Agilent Parallel Capillary Electrophoresis Instruments

Created by Jasmin Manck-Goetzenberger Jasmin Manck-Goetzenberger over 2 years ago | Last modified by Shweta.S Shweta.S 10 months ago

This Information Applies To: 5200/5300/5400 Fragment Analyzer, Femto Pulse, Oligo Pro II, ZAG, and their respective controller software


Issue:

A full power cycle can help to recover communication/connection issues of Agilent parallel capillary electrophoresis instruments (5200/5300/5400 Fragment Analyzer, Femto Pulse, Oligo Pro II, and ZAG systems). Communication and connection issues can materialize already during software start as connection failed error messages although the instrument is powered on and connected to the PC (see Figure1).

 
Figure 1. Examples of connection error messages

 Tip  Also the error Run aborted, Error moving syringe plunger might be resolved by a full power cycle. For more information, see here.

Steps to follow:  

  1. Exit any open programs (controller software, ProSize, etc.).

  2. Power off both the instrument and PC (see instrument power switch in Figure 2, A).



    Figure 2. Example back panel with instrument connections of the Fragment Analyzer instrument (A and B), and of the PC (C). All relevant parts for the full power cycle are marked.
    1. Labeled USB connections, 2. Fuse mount, 3. Power switch, 4. AC power connection, 5. Internet connection, 6. Computer monitor connection


  3. Unplug the instrument and PC power cables from the wall socket before unplugging the cables from the back of the instrument and PC (see AC power connections in Figure 2, A-C).

  4. Unplug the two USBs labeled CCD and HUB from the back of the PC (see Figure 2, C).

  5. Wait for 10 minutes.

  6. Reconnect USB cables to different, nonadjacent ports and reconnect power cables to the instrument and PC.

  7. Power on the PC and instrument and re-open the controller software.

 Tip  Learn how to effectively operate your Agilent parallel capillary electrophoresis system:
5200, 5300, and 5400 Fragment Analyzer System Manual
Femto Pulse User Manual
Oligo Pro II User Manual 
ZAG DNA Analyzer System Manual 

For Research Use Only. Not for use in diagnostic procedures.

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