Agilent Community
Agilent Community
  • User
  • Site
  • Search the Community
  • User
Automated Electrophoresis Portal
  • Agilent Knowledge Portal
Automated Electrophoresis Portal
Automated Electrophoresis Articles Reservoir Vent Valve Cleaning for Parallel Capillary Electrophoresis Instruments
  • Automated Electrophoresis Articles
  • Automated Electrophoresis Forum
  • More
  • Cancel

Browse the Agilent Knowledge Portal

  • Chromatography
    • Gas Chromatography
    • Liquid Chromatography
  • More
  • Cancel
  • GC/MS
  • More
  • Cancel
  • Spectroscopy
    • Atomic Absorption
    • ICP-OES
  • More
  • Cancel
  • Automated Electrophoresis
  • More
  • Cancel
  • Software and Informatics
    • OpenLab CDS
    • MassHunter
  • More
  • Cancel
  • -Automated Electrophoresis
    • +Bioanalyzer
    • +Tapestation
    • -Parallel Electrophoresis
      • Failed to Build Pressure Error on a Parallel Capillary Electrophoresis Instrument
      • Run Aborted and Target Z Position Error on Parallel Capillary Instruments
      • Analysis of Single Stranded DNA on the Fragment Analyzer
      • How to do a NaOH Flush on an Agilent Fragment Analyzer
      • Agilent Fragment Analyzer Message: Setup Incorrect - Ladder File does not Exist
      • Agilent Fragment Analyzer or Femto Pulse: Run Aborted, Error Moving Syringe Plunger
      • Agilent Parallel Electrophoresis Capillary Array Disposal
      • Re-ordering the Syringe for Cleaning the Reservoir Vent Valve of the Agilent Fragment Analyzer, Femto Pulse, or ZAG
      • DV200 Analysis of FFPE RNA Samples with the Fragment Analyzer
      • Agilent Capillary Array Installation on Parallel Capillary Electrophoresis Instruments
      • Error 97 When Creating PDF Report in ProSize Data Analysis Software
      • How to Clean the Capillary Array Window on Agilent Parallel Capillary Electrophoresis Instruments
      • Reservoir Vent Valve Cleaning for Parallel Capillary Electrophoresis Instruments
      • Full Power Cycle for Agilent Parallel Capillary Electrophoresis Instruments
      • Agilent Parallel Capillary Electrophoresis Compatible Plasticware
      • How to Use Mineral Oil to Prevent Evaporation on Agilent Parallel Capillary Electrophoresis Systems
      • Running Dye Labeled Samples on Agilent Parallel Capillary Electrophoresis Instruments
      • Insert Disk Error in the Agilent Fragment Analyzer Controller Software
      • BAC Method Missing from the Agilent Femto Pulse Software

Reservoir Vent Valve Cleaning for Parallel Capillary Electrophoresis Instruments

Created by Jasmin Manck-Goetzenberger Jasmin Manck-Goetzenberger over 1 year ago | Last modified by Shweta.S Shweta.S 1 month ago

This Information Applies To: Fragment Analyzer, Femto Pulse, ZAG, and their respective controller software


Issue:

If there is dried gel in the reservoir vent valve of your Agilent Fragment Analyzer, Femto Pulse, or ZAG, cleaning is required. A clogged reservoir vent valve can impact the separation performance, for example leading to delayed migration. 

 Tip Recommended maintenance schedule:  Agilent recommends inspecting the vent valve for clogs as part of troubleshooting procedures and your monthly maintenance. Clean if necessary. For more information on maintenance, please see the preventative maintenance schedule in the respective user manual.


Steps to follow

  1. From the Utilities menu in the controller software, select Clean Reservoir Vent Valve. The actual reservoir vent valve opens for cleaning and also the Clean Reservoir Vent Valve dialog box will be shown on screen (see Figure 1). 
     
    Figure 1. How to open the Clean Reservoir Vent Valve dialog box
  2. Use the 10 mL Luer lock syringe to:
    1. either follow the onscreen instructions and clean the reservoir vent valve sequentially with 7 mL of DI water and 10 mL of air,
    2. or for a more thorough cleaning to remove severe clogs, clean it five times with 7 mL of 0.5 N NaOH, followed by 7 mL of DI water and then 10 mL air.
  3. For the cleaning steps, attach the filled 10 mL syringe to the Luer lock fitting of the reservoir vent valve as shown in Figure 2 and press down the plunger.

 

Figure 2. Emptied syringe indicated with red box attached to the reservoir vent valve of a Fragment Analyzer

In case you need to re-order the 10 mL syringe, please use any 10 mL Luer Lock tip plastic syringe (not sold via Agilent Technologies). The Luer lock tip is compatible to the reservoir vent valve.

 

Tip
Learn how to effectively maintain your Agilent parallel capillary electrophoresis instruments:
Fragment Analyzer system manual
Femto Pulse system manual
ZAG system manual

For Research Use Only. Not for use in diagnostic procedures.

PR7000-8101

  • ZAG DNA Analyzer
  • Troubleshooting
  • Femto Pulse
  • Maintenance
  • Parallel Electrophoresis
  • Fragment Analyzer Systems
  • Fragment Analyzer
  • Femto Pulse System
  • Share
  • History
  • More
  • Cancel
Was this helpful?
  • Yes
  • No
Thank you for your feedback!

Agilent Community Feedback

Agilent Community Feedback

×
We are glad this was helpful! We are sorry this was not helpful. If you still need assistance please create a community post or contact support. To help us improve, please provide any additional feedback. For full details of how we will treat your information, please view our privacy policy.
Submit Cancel
Submit Cancel

Contact Agilent Support

 Still Need Assistance?

 Contact Agilent Support >

Related
Recommended for you
Privacy Statement
Terms of Use
Contact Us
Site Help